Point-of-Care Optical Imaging Technologies

Review Paper:

• S. A. Boppart, R. Richards-Kortum, Point-of-care and point-of-procedure optical imaging technologies for primary care and global health. Sci. Transl. Med. 6, 253rv2 (2014).

“This paper reviewed the latest development of optical methods for point-of-care and discussed its opportunities and challenges.”

Related products/research

1. LED-based Fluorescence microscope adaptor to bright-field microscope. Foundation for Innovative New Diagnostics. It has done field test in Uganda. Normal version costs around 700-1750 USD, and low-cost, battery-powered version costs around 240usd.

   • H. Albert, Y. Manabe, G. Lukyamuzi, P. Ademun, S. Mukkada, B. Nyesiga, M. Joloba, C. N. Paramasivan, M. D. Perkins, Performance of three LED-based fluorescence microscopy systems for detection of tuberculosis in Uganda. PLOS One 5, e15206 (2010).

   • A. R. Miller, G. L. Davis, Z. M. Oden, M. R. Razavi, A. Fateh, M. Ghazanfari, F. Abdolrahimi, S. Poorazar, F. Sakhaie, R. J. Olsen, A. R. Bahrmand, M. C. Pierce, E. A. Graviss, R. Richards-Kortum, Portable, battery-operated, low-cost, bright field and fluorescence microscope. PLOS One 5, e11890 (2010).

2. Modular, portable fluorescence microscope for white blood cell count.

   • Majors, Catherine E., et al. “Low-cost disposable cartridge for performing a white blood cell count and partial differential at the point-of-care.” Healthcare Innovation Conference (HIC), 2014 IEEE. IEEE, 2014.

3. Mobile phone-based clinical microscopy.

   • D. N. Breslauer, R. N. Maamari, N. A. Switz, W. A. Lam, D. A. Fletcher, Mobile phone based clinical microscopy for global health applications. PLOS One 4, e6320 (2009).

   • A. Skandarajah, C. D. Reber, N. A. Switz, D. A. Fletcher, Quantitative imaging with a mobile phone microscope. PLOS One 9, e96906 (2014).

4. An ultra-low-cost, origami-based bright-field, dark-field and fluorescence microscope, “Foldscope”

   • J. S. Cybulski, J. Clements, M. Prakash, Foldscope: Origami-based paper microscope. PLOS One 9, e98781 (2014).

5. A lens-free cell phone holographic microscope.

   • A. Greenbaum, W. Luo, T. W. Su, Z. Göröcs, L. Xue, S. O. Isikman, A. F. Coskun, O. Mudanyali, A. Ozcan, Imaging without lenses: Achievements and remaining challenges of wide-field on-chip microscopy. Nat. Methods 9, 889–895 (2012).

   • A. F. Coskun, A. Ozcan, Computational imaging, sensing and diagnostics for global health applications. Curr. Opin. Biotechnol. 25, 8–16 (2014).

6. Flow-based systems: cell phone imaging-based flow cytometry

   • H. Zhu, S. Mavandadi, A. F. Coskun, O. Yaglidere, A. Ozcan, Optofluidic fluorescent imaging cytometry on a cell phone. Anal. Chem. 83, 6641–6647 (2011).

   • W. G. Lee, Y. G. Kim, B. G. Chung, U. Demirci, A. Khademhosseini, Nano/microfluidics for diagnosis of infectious diseases in developing countries. Adv. Drug Deliv. Rev. 62, 449–457 (2010).

   • N. N. Watkins, U. Hassan, G. Damhorst, H. Ni, A. Vaid, W. Rodriguez, R. Bashir, Microfluidic CD4+ and CD8+ T lymphocyte counters for point-of-care HIV diagnostics using whole blood. Sci. Transl. Med. 5, 214ra170 (2013).

   • G. Zheng, S. A. Lee, S. Yang, C. Yang, Sub-pixel resolving optofluidic microscope for onchip cell imaging. Lab Chip 10, 3125–3129 (2010).

   • S. A. Lee, R. Leitao, G. Zheng, S. Yang, A. Rodriguez, C. Yang, Color capable sub-pixel resolving optofluidic microscope and its application to blood cell imaging for malaria diagnosis. PLOS One 6, e26127 (2011).

   • S. A. Lee, J. Erath, G. Zheng, X. Ou, P. Willems, D. Eichinger, A. Rodriguez, C. Yang, Imaging and identification of waterborne parasites using a chip-scale microscope. PLOS One 9,e89712 (2014).

7. Handheld OCT

   • W. Jung, J. Kim, M. Jeon, E. J. Chaney, C. N. Stewart, S. A. Boppart, Handheld optical coherence tomography scanner for primary care diagnostics. IEEE Trans. Biomed. Eng. 58, 741–744 (2011).

   • R. L. Shelton, W. Jung, S. I. Sayegh, D. T. McCormick, J. Kim, S. A. Boppart, Optical coherence tomography for advanced screening in the primary care office. J. Biophotonics 7, 525–533 (2014).

   • C. T. Nguyen, W. Jung, J. Kim, E. J. Chaney, M. Novak, C. N. Stewart, S. A. Boppart, Noninvasive in vivo optical detection of biofilm in the human middle ear. Proc. Natl. Acad. Sci. U.S.A. 109, 9529–9534 (2012).